acta2-CatCh-IRES-lacZ Strain Information Sheet
Common Name: acta2-CatCh-IRES-lacZ
CHROMusTM designation and lines available: R24:B8 Line 3
Jackson Stock number: NA
Development: The rhodopsin based effector, CatCh was inserted at the ATG site of the acta2 gene in the BAC RP23-370F21 (CHORI) through homologous recombination. The resulting recombinant BAC was injected into the male pronucleus of fertilized oocytes which were then implanted into pseudo pregnant females. Resulting offspring were screened for the presence of the transgene (founders). Colonies were established from each founder and tested for expression.
|# pups born||# founders||# expressers|
Description: The rhodopsin based effector, CatCh is expressed under control of the acta2 promoter, directing expression to smooth muscle cells in blood vessels, airways of the lung and gut. Expression may be present in other smooth muscle containing tissues which have not been examined. An internal ribosome entry site (IRES) allows for co-expression of lacZ as a visual indicator of expression patterns.
Photo-activation of CatCh. Blue light is flashed at t=6 seconds, inducing vasoconstriction in vascular smooth muscle. See screenshots below.
|Primer B119||GCT TGT CTG TAA GCG GAT GCC|
|Primer B120||TGC TGC TGC CAC TCT AGT GAG AAA|
|Expected size of product:||602bp|
94oC 3 minutes
Acknowledgement: Please inform us of any publications resulting from the use of this mouse and use the following statement to acknowledge their development:
“The mouse strain acta2-CatCh-IRES-lacZ was developed by CHROMusTM; funded by NIH R24HL120847.”