B5

αMHC-CatCh-IRES-lacZ Strain Information Sheet

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Strain Name:

Common Name: αMHC-CatCh-IRES-lacZ

CHROMusTM designation and lines available: R24:B5

Jackson Stock number: NA

Development: The rhodopsin based effector, CatCh was inserted under control of the αMHC minimal promoter (Addgene 55594). The resulting transgene was injected into the male pronucleus of fertilized oocytes which were then implanted into pseudo pregnant females. Resulting offspring were screened for the presence of the transgene (founders). Colonies were established from each founder and tested for expression.

Transgenic Numbers:

# pups born # founders # expressers
24 8 3

Description: The rhodopsin based effector, CatCh is expressed under control of the αMHC promoter, directing expression to cardiomyocytes in the heart.   An internal ribosome entry site (IRES) allows for co-expression of lacZ as a visual indicator of expression patterns.

Phenotypic Data:

qPCR of CatCh message in the heart.

qPCR of CatCh message in the heart.

lacZ staining (frozen section) of heart from line 10.

lacZ staining (frozen section) of heart from line 10.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Additional data detailing CatCh activity will be added.

Genotyping Protocol:

Primer B107 GGA GAT CTA TGT GTG CGC TAT C
Primer B108 CCA GCA GTT CTT CGA CAT CA
Expected size of product: 535bp

Cycling conditions:

94oC 3 minutes
Repeat 30 times
30 sec @ 94oC
30 sec @ 58oC
30 sec @ 72oC
72oC 5 minutes
10oC hold

Terms of Use: Please contact chromus@cornell.edu for information on MTA and fees.

Acknowledgement: Please inform us of any publications resulting from the use of this mouse and use the following statement to acknowledge their development:

“The mouse strain αMHC-CatCh-IRES-lacZ was developed by CHROMusTM; funded by NIH R24HL120847.”

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