B16

dbh-CatCh-IRES-lacZ Strain Information Sheet

indicate interest

Strain Name:

Common Name: dbh-CatCh-IRES-lacZ

CHROMusTM designation and lines available: R24:B16

Jackson Stock number: NA

Development: The rhodopsin based effector, CatCh was inserted at the ATG site of the dbh gene in the BAC RP23-354N13 (CHORI) through homologous recombination. The resulting recombinant BAC was injected into the male pronucleus of fertilized oocytes which were then implanted into pseudo pregnant females. Resulting offspring were screened for the presence of the transgene (founders). Colonies were established from each founder and tested for expression.

Transgenic Numbers:

# pups born # founders # expressers
26 7 1

Description: The rhodopsin based effector, CatCh is expressed under control of the dbh promoter, directing expression to sympathetic neurons and chromaffin cells of the adrenal medulla. An internal ribosome entry site (IRES) allows for co-expression of lacZ as a visual indicator of expression patterns.  NOTE: lacZ expression is appropriate, functional analysis of the CatCh cassette is ongoing.

Phenotypic Data:

Cells in the brain (left) and adrenal medulla (right) expressing lacZ expressed under control of the dbh promoter.

 

Genotyping Protocol:

Primer B107 GGA GAT CTA TGT GTG CGC TAT C
Primer B108 CCA GCA GTT CTT CGA CAT CA
Expected size of product: 535bp

Cycling conditions:

94oC 3 minutes
Repeat 30 times
30 sec @ 94oC
30 sec @ 58oC
30 sec @ 72oC
72oC 5 minutes
10oC hold

 

Terms of Use: Please contact chromus@cornell.edu for information on MTA and fees.

So as to appropriately acknowledge and credit the scientists who have produced the dbh-CatCh-IRES-lacZ mouse strain, it is expected that you will include these individuals in any publication that is submitted prior to the initial publication of this line from the Kotlikoff laboratory.

Acknowledgement: Please inform us of any publications resulting from the use of this mouse and use the following statement to acknowledge their development:

The mouse strain dbh-CatCh-IRES-lacZ was developed by CHROMusTM which is supported by the National Heart Lung Blood Institute of the National Institute of Health under award number R24HL120847.

 

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