HCN4-GCaMP8 Strain Information Sheet
Common Name: HCN4-GCaMP8
CHROMusTM designation and lines available: R24:B10:L3
Jackson Stock number: NA
Development: The green fluorescent calcium indicator GCaMP8 was inserted at the ATG site of the HCN4 gene in the BAC RP23-281H22 (CHORI) through homologous recombination. The resulting recombinant BAC was injected into the male pronucleus of fertilized oocytes which were then implanted into pseudo pregnant females. Resulting offspring were screened for the presence of the transgene (founders). Colonies were established from each founder and tested for expression.
|# pups born||# founders||# expressors|
Description: The genetically encoded calcium indicator GCaMP8 is expressed under control of the HCN4 promoter, directing expression to the SA node and the AV node of the heart and the nervous system (brain and spinal cord). Expression is observed in the developing 13.5 dpc heart and in the brain and spinal cord. GCaMP8 responds to calcium levels in the cell. When calcium increases, a conformation change occurs resulting in an increase in fluorescence. When calcium decreases, fluorescence decreases. This mouse is useful for examining calcium signaling in the SA and AV nodes.
Figure 4: calcium signaling in the SA node of a 13.5 dpc embryo.
|Primer CalmF||AAG GGC GAG GAG CTG TTC A|
|Primer CalmR||CGA TCT GCT CTT CAG TCA GTT GGT|
|Expected size of product:||456bp|
Acknowledgement: Please inform us of any publications resulting from the use of this mouse and use the following statement to acknowledge their development:
“The mouse strain HCN4-GCaMP8 was developed by CHROMusTM; funded by NIH R24HL120847.”