Mouse Production

  Transgenic Mouse Production

(click on any tissue system or line for more details)

For details on the construction of transgenes, click here.

In production
Analysis and confirmation
Smooth muscle system (acta2 promoter):
B6: acta2-Optoa1AR-IRES-lacZ

 request mice
B7: acta2-Optob2AR-IRES-lacZ  √  √ request mice
B8: acta2-CatCh-IRES-lacZ  √  √ request mice
B3: acta2-RCaMP1.07  √  √ request mice
B19: acta2-GCAMP2*  √  √  Jax# 25405
B14: acta2-GCaMP-GR*  √  √   Jax# 25406
B25: acta2-GCaMP-ER  √
B34: acta2-GCaMP8.1-mVermilion  √  √  request mice
 Cardiac and venous system (αMHC, HCN4, Cdh5, mesp1, Cx40 and POSTN promoters):
B5: αMHC-CatCh-IRES-lacZ  √  request mice
B4: αMHC-GCaMP8 request mice
B17: HCN4-CatCh-IRES-lacZ  request mice
B10: HCN4-GCaMP8 request mice
B20: Cdh5-GCaMP8  √ request mice
B26: Cdh5-Optoα1AR-IRES-lacZ  √  request mice
B27: Cdh5-optoβ2AR-IRES-lacZ  request mice
B28: Cdh5-CatCh-IRES-lacZ  request mice
B29: Cdh5-GCaMP8-mVermillion
mesp1-mCherry*  Jax# 25618
Cx40-GCaMP2*  Jax# 25404
B15: Cx40-GCaMP-GR* request mice
B31: POSTN-CatCh-IRES-lacZ
Nervous system (DBH, ChAT, gfap and Aldh1L1 promoters):
B12: ChAT-GCaMP8
B13: dbh-GCaMP8
B16: dbh-CatCh-IRES-lacZ
B11: gfap-GCaMP-GR*
B21: CGRP-GCaMp8
B36: Aldh1L1-optoα1AR-IRES-lacZ
Widespread expression:
 pCAGGS-GCaMP2*  JAX# 25619
Immune system (Lck promoter):
B22: Lck-optoα1AR-IRES-lacZ
B32: Lck-GCaMP8
B37: Ly6G-optoα1AR-IRES-mVermilion
B38: Ly6G-optoβ2AR-IRES-mVermilion
Lung Epithelium (pdpn, SP-C, FoxJ1 and CCSP promoters):
B23: SP-C-GCaMP8  √ request mice
B24: pdpn-GCaMP8
B35: FoxJ1-GCaMP8.1
*previously developed in the Kotlikoff Laboratory


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