Shui B, Lee JC, Reining S, Lee FK, Kotlikoff MI.  CHROMus: the Cornell/National Heart Lung Blood Institute Resource for optogenetic mice.  Front. Physiol., 06 November 2014 | doi: 10.3389/fphys.2014.00428

Genetically Encoded Calcium Indicators:

Wang Q, Shui B, Kotlikoff MI, Sondermann H. Structural basis for calcium sensing by GCaMP2. Structure. 2008 Dec 10;16(12):1817-27.

Kotlikoff MI. Genetically encoded Ca2+ indicators: using genetics and molecular design to understand complex physiology. J Physiol 2007 Jan 1;578(Pt 1):55-67

Muller-Taubenberger A. Application of fluorescent protein tags as reporters in live-cell imaging studies. Methods Mol.Biol. 2006;346:229-46


Prigge M, Schneider F, Tsunoda SP, Shilyansky C, Wietek J, Deisseroth K, Hegemann P. Color-tuned channelrhodopsins for multiwavelength optogenetics. J Biol.Chem. 2012 Sep 14;287(38):31804-12

Fenno L, Yizhar O, Deisseroth K. The development and application of optogenetics. Annu.Rev.Neurosci. 2011;34:389-412

Zhang F, Wang LP, Boyden ES, Deisseroth K. Channelrhodopsin-2 and optical control of excitable cells. Nat.Methods 2006 Oct;3(10):785-92

Boyden ES, Zhang F, Bamberg E, Nagel G, Deisseroth K. Millisecond-timescale, genetically targeted optical control of neural activity. Nat.Neurosci. 2005 Sep;8(9):1263-8

Kleinlogel S, Feldbauer K, Dempski RE, Fotis H, Wood PG, Bamann C, Bamberg E.  Ultra light-sensitive and fast neuronal activation with the Ca(2)+-permable channelrhodopsin CatCh.  Nat. Neurosci. 2011 14:513-518.

BAC recombineering:

Warming S, Costantino N, Court DL, Jenkins NA, Copeland NG. Simple and highly efficient BAC recombineering using galK selection. Nucleic Acids Res. 2005;33(4):e36

Liu P, Jenkins NA, Copeland NG. A highly efficient recombineering-based method for generating conditional knockout mutations. Genome Res. 2003 Mar;13(3):476-84

Lee EC, Yu DG, de Velasco JM, Tessarollo L, Swing DA, Court DL, Jenkins NA, Copeland NG. A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA. Genomics 2001 Apr 1;73(1):56-65

Mice Developed in Dr. Kotlikoff’s laboratory:mike_kotlikoff_and_friend_76672.tif

Nausch LW, Bonev AD, Heppner TJ, Tallini Y, Kotlikoff MI, and Nelson MT.  Sympathetic nerve stimulation induces local endothelial Ca2+ signals to oppose vasoconstriction of mouse mesenteric arteries.  Am J Physiol Heart Circ Physiol. 302(3):  H594-602, 2012.

Krasteva G, Canning BJ, Hartmann P, Veres TZ, Papadakis T, Mühlfeld C, Schliecker K, Tallini YN, Braun A, Hackstein H, Baal N, Weihe E, Schütz B, Kotlikoff M, Ibanez-Tallon I, and Kummer W.  Cholinergic chemosensory cells in the trachea regulate breathing.  PNAS 108(23):  9478-83, 2011.

Schnitzler AC, Mellott TJ, Lopez-Coviella I, Tallini YN, Kotlikoff MI, Follettie MT, and Blusztajn JK. BMP9 (bone morphogenetic protein 9) induces NGF as an autocrine/paracrine cholinergic trophic factor in developing basal forebrain neurons. J Neuroscience  30(24):  8221-8, 2010.

Nelson M, Ledoux J, Taylor M, Bonev A, Hannah R, Solodushko V, Shui B, Tallini Y, and Kotlikoff M.  Spinning Disk Confocal Microscopy of Calcium Signalling in Blood Vessel Walls.  Microsc Anal (Am Ed) 24(2):  5-8, 2010.

Tallini YN, Greene KS, Craven M, Spealman A, Breitbach M, Smith J, Fisher PJ, Steffey M, Hesse M, Doran RM, Woods A, Singh B, Yen A, Fleischmann BK, Kotlikoff MI. c-kit expression identifies cardiovascular precursors in the neonatal heart. Proc.Natl.Acad.Sci.U.S.A 2009 Feb 10;106(6):1808- 13

Ledoux J, Taylor MS, Bonev AD, Hannah RM, Solodushko V, ,Shui B, Tallini YN, Kotlikoff MI, and Nelson MT. Functional architecture of inositol 1,4,5-trisphospate signaling in restricted spaces of myoendothelial projections. PNAS 105: 9627-9632, 2008.

Roell W, Lewalter T, Choi B, Tallini YN, Bostani T, Becher U, Sasse P, Hwang S, Doran R, Breitbach M, Reining S, Gabris B, Welz A, Salama G, Schrickel J, Kotlikoff MI, Fleischmann B. Engraftment of connexin 43-expressing cells prevents post-infarct arrhythmia.  Nature 450: 819-824, 2007.

Ogura T, Margolskee RF, Tallini YN, Shui B, Kotlikoff M, Lin W.  Immuno-localization of Vesicular Acetylcholine Transporter in Mouse Taste Cells and Adjacent Nerve Fibers:  Indication of Acetylcholine Release. Cell & Tissue Res 330: 17-28, 2007.

Tallini YN, Brekke JF, Shui B, Doran R, Hwang SM, Nakai J, Salama G, Segal SS, Kotlikoff MI. Propagated endothelial Ca2+ waves and arteriolar dilation in vivo: measurements in Cx40BAC GCaMP2 transgenic mice. Circ.Res. 2007 Dec 7;101(12):1300-9

Tallini YN, Shui B, Greene KS, Deng KY, Doran R, Fisher PJ, Zipfel W, Kotlikoff MI. BAC transgenic mice express enhanced green fluorescent protein in central and peripheral cholinergic neurons. Physiol Genomics 2006 Nov 27;27(3):391-7

Tallini YN, Ohkura M, Choi BR, Ji G, Imoto K, Doran R, Lee J, Plan P, Wilson J, Xin HB, Sanbe A, Gulick J, Mathai J, Robbins J, Salama G, Nakai J, Kotlikoff MI. Imaging cellular signals in the heart in vivo: Cardiac expression of the high-signal Ca2+ indicator GCaMP2. Proc.Natl.Acad.Sci.U.S.A 2006 Mar 21;103(12):4753-8

Kotlikoff, MI and Tallini NY.  Imaging Dynamic Cellular Events in the Lung. J Gen Phys 125:  529-530, 2005.

Ji G, Feldman ME, Deng KY, Green KS, Wilson J, Lee JC, Johnston RC, Rishniw M, Tallini Y, Zhang J, Wier WG, Blaustein MP, Xin HB, Nakai J, Kotlikoff MI.  Ca2+-sensing Transgenic Mice.  J Biol Chem 279:  21461-21468, 2004.

Xin HB, Deng KY, Rishniw M, Ji G, Kotlikoff MI. Smooth muscle expression of Cre recombinase and eGFP in transgenic mice. Physiol Genomics 2002 Sep 3;10(3):211-5

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