Cardiac and Venous system

αMHC (α myosin heavy chain) directing expression to cardiomyocytes:

Using standard cloning techniques, the sensor and effector cassettes are placed under control of the minimal αMHC promoter, directing expression to cardiomyocytes.   The promoter is a 5.5kb fragment including the 3′ most exon of βMHC, the first three exons of αMHC (up to the start codon) as well as intervening sequences. For additional information click here.

αMHC – GCaMP8

αMHC – CatCh-IRES-lacZ

αMHC – RCaMP1.07 (future construct)

αMHC – ER-GCaMP1 (future construct)

αMHC – Opto-α1AR-IRES-lacZ (future construct)

αMHC – Opto-β2AR-IRES-lacZ (future construct)

HCN4 (hyperpolarization activated cyclic nucleotide-gated potassium channel) directing expression to the SA node and the AV node:

The sensor and effector cassettes are under control of the HCN4 promoter, directing expression to the SA node and the AV node.  Using homologous recombination in BAC RP23-281H22, the initiation codon of HCN4 was replaced with the expression cassette.  For construction details, click here.

HCN4 – GCaMP8

HCN4 – CatCh-IRES-lacZ

HCN4 – RCaMP1.07 (future construct)

HCN4 – ER-GCaMP1 (future construct)

HCN4 – Opto-α1AR-IRES-lacZ (future construct)

HCN4 – Opto-β2AR-IRES-lacZ (future construct)

Cdh5 (VE-cadherin) directing expression to vascular endothelial cells:

The sensor and effector cassettes are under control of the cdh5 promoter, directing expression to vascular endothelial cells.  Using homologous recombination in BAC RP23-453P1, the initiation codon of cdh5 was replaced with the expression cassette.  For construction details, click here.

cdh5-GCaMP8

cdh5-optoa1AR-IRES-lacZ

cdh5-optob2AR-IRES-lacZ

cdh5-CatCh-IRES-lacZ

cdh5-GCaMP8-mVermilion