Examples of signaling constructs and mice made in the Kotlikoff lab:
Click on an image for full size:
Hela cells expressing mCherry in the cytoplasm and GCaMP in the ER (Kotlikoff, unpublished data)
Arteriolar endothelial cell expression of GCAMP2
(Tallini et al Circ.Res. 2007; 101(12):1300-9)
Cholinergic nerves expressing EGFP in lung, stomach and intestine
(Tallini et al, Physiol Genomics 2006 Nov 27;27(3):391-7)
Cardiac cells expressing GcaMP2
(Tallini et al PNAS 2006;103(12):4753-8)
GCaMP2 staining in smooth muscle of the myocardium (left) and bronchus (right)
(Kotlikoff, unpublished)
In vivo demonstration of calcium signaling using GCaMP2:
Ca2+–dependent fluorescence (GCaMP2) in cardiomyocytes during a single cardiac cycle in an anesthetized and ventilated mouse.
(Tallini et al, PNAS 2006 Mar 21;103(12):4753-8)
8 day mouse heart activating from the atrium (first beat) but with a premature ventricular depolarization also occurring. In the last depolarization the activation is retrograde and slows in the AV canal.
In vivo imaging of an arteriole in the mouse cremaster muscle showing dilation of the vessel after application of acetylcholine
In vivo monitoring of functional engraftment of GCaMP2+ embryonic cardiomyocytes in an infarct. (Roell et al, Nature 450: 819-824, 2007)
Mouse ES cells expressing GCaMP2 and differentiated to cardiomyocytes. (Kotlikoff, unpublished data)
Isolated embryonic cardiomyocytes spontaneously beating in culture.
Ca2+ Signaling in the developing heart of a 10.5 day embryo during spontaneous beating. Note slow passage of Ca2+ wave through the AV canal (AVC) and progression into ventricle. (Tallini et al, PNAS 2006;103(12):4753-8)
In vivo imaging of mouse embryos expressing Cx40-GCaMP2 in the heart. (Kotlikoff, unpublished data)
Adult mouse perfused heart expressing GCaMP2 showing calcium levels